A podcast about computational biology, bioinformatics, and next generation sequencing.
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episode 9: Michael Tessler and Christopher Mason on 16S amplicon vs shotgun sequencing
Michael Tessler and Christopher Mason join me to talk about their comparison of 16S amplicon sequencing and shotgun sequencing for quantifying microbial diversity.
Links:
- The 2017 Nature paper that we discuss: Large-scale differences in microbial biodiversity discovery between 16S amplicon and shotgun sequencing
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Michael’s et al. 2016 paper that describes their original 16S study: A Global eDNA Comparison of Freshwater Bacterioplankton Assemblages Focusing on Large-River Floodplain Lakes of Brazil
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The sequencing data for these studies is available from NCBI: PRJNA310230 (16S), PRJNA389803 (shotgun)
- Michael’s website
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Christopher’s lab website
- The Integrative Biology & Medicine conference, featuring the talk on Postmodern Synthesis by Eugene Koonin
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