the bioinformatics chat

I talk about allele-specific expression: why it arises and how it can be reliably detected.

Sections:

• The biology of allele-specific expression (2:17)
• Detecting allele-specific expression with RNA-seq (7:46)
• Mapping and sequencing biases (16:39)
• The experiment in yeast (19:47)
• Statistical models (21:44)

Links:

• A powerful and flexible statistical framework for testing hypotheses of allele-specific gene expression from RNA-seq data
  • Supplemenal information
• Effect of read-mapping biases on detecting allele-specific expression from RNA-sequencing data
• The talk by John Marioni
• The blog post explaining the RSEM model

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