A podcast about computational biology, bioinformatics, and next generation sequencing.
https://bioinformatics.chat
episode 27: Classification of CRISPR-induced mutations and CRISPRpic with HoJoon Lee and Seung Woo Cho
In this episode, HoJoon Lee and Seung Woo Cho explain how to perform a CRISPR experiment and how to analyze its results. HoJoon and Seung Woo developed an algorithm that analyzes sequenced amplicons containing the CRISPR-induced double-strand break site and figures out what exactly happened there (e.g. a deletion, insertion, substitution etc.)
Links:
- CRISPRpic: Fast and precise analysis for CRISPR-induced mutations via prefixed index counting
- CRISPRpic on GitHub
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