ProUCL version 5.1.002 (5.1) is the latest update of the ProUCL statistical software package for analysis of environmental data sets with and without nondetect (ND) observations. The ProUCL software is available at https://www.epa.gov/land-research/proucl-software. This second installment of the three part ProUCL e-learning seminars will be presenting live interactive use of ProUCL v5.1 focussing specifically on the finer points of regression and trend analysis within the ProUCL software. General discussion points will includeData ManagementOLS RegressionMann-Kendall Trend TestTheil-Sen Line TestTime Series AnalysisA ProUCL provided trend dataset (XLS), trend dataset with additional variables (XLS), and dataset to show time series plots with grouping variable (XLS) will be used as an example throughout the presentation. This datasets are provided here and it is encouraged for attendees to follow along on their local machine. To view this archive online or download the slides associated with this seminar, please visit http://www.clu-in.org/conf/tio/ProUCLAtoZ2_021020/