Gene Editing with CRISPR/Cas
September 14, 2018
(duration 1h31m)
Guest: Sam Sternberg Host: Markus Voelter Shownoter: Thomas Machowinski
CRISPR is a family of DNA sequences in bacteria and archaea that are a part of these organisms’ cellular defense system. A recent discovery showed how this mechanism can be used to edit genes much more easily than legacy methods. In this episode I chat with Sam Sternberg about the naturally occuring CRISPR systems, how they work, and how CRISPR together with its associated enzymes can be used to cut, and subsequently, edit, DNA. We conclude the episode with an outlook on the potential use in medicine.
Check out Sam’s book A Crack in Creation: Gene Editing and the Unthinkable Power to Control Evolution.
Es gibt außerdem ein Kurz&Knackig Video:
Intro and General 00:02:22
Guest is Samuel Sternberg | CRISPR Clustered Regularly Interspaced Short Palindromic Repeats | CAS9 CRISPR Associated protein 9 | DNA | Gene | RNA | Protein | Enzymes | 2007 Discovery: CRISPR gene sequences stores DNA information from pathogenes / e.g. viruses
Genome Editing with CRISPR/CAS9 00:23:02
Genome Editing | Nucleus | ZFN | TALEN | 23andMe | Homolgy directed repair | Transfection | Off-target effect | GMO | Genome splicing | CRISPR/CAS9 Method in comparsion to traditional genome editing and breeding
Genral Discussion 01:09:45
Patents in Biology | Sam's work in detail | Cas13 edits RNA instead of DNA | Sam's book
Engineering ‘Selfish’ Genes in Mice: Benefits and Risks - Exploring Ethics
June 29, 2018
(duration 57m)
Kim Cooper, Assistant Professor in the Cell and Developmental Biology Section of the UC San Diego Division of Biological Sciences, discusses the advantages and the potential risks of CRISPR/Cas9-based active genetic systems and ways to maximize benefits to society. How do we decide when not to do the things that we can? In the last couple of years, a new “active genetic” technology has been shown to promote efficient inheritance of desired gene modifications in insects. We have now shown that we can do this in rodents. This allows the assembly of complex genotypes that were once unthinkable due to cost, time, and more. Such applications could improve drug testing and mouse models of complex human genetic diseases. These same approaches could also be used to control invasive wild rodent populations and vectors of disease. Despite these benefits, many have raised concerns about unintended consequences of the release of transgenic organisms. Series: "Exploring Ethics" [Show ID: 33672]
Jennifer Doudna in Conversation with Joe Palca
February 6, 2018
(duration 58m)
Hear about Jennifer Doudna's discovery of CRISPR/Cas9, a genome editing tool, and its bioethical implications. Doudna, Professor of Molecular & Cell Biology and Chemistry at UC Berkeley, speaks with NPR's Joe Palca. Series: "Lawrence Berkeley National Laboratory " [Show ID: 33095]
Editing the Genome - Part Two
March 14, 2016
(duration 27m)
There is a new genetic technology which promises to revolutionise agriculture and transform our influence over the natural world. Research is well underway to create pigs and chickens immune to pandemic influenza, cereals which make their own fertiliser and mosquitoes engineered to wipe out wild populations of the insects which transmit diseases to humans. These are just three examples of what we could create with CRISPR gene editing.
Should we be worried about this unprecedented power over animals and plants? The potential for good is enormous. The ethical challenges are profound. Professor Matthew Cobb of the University of Manchester explores the brave new world of CRISPR gene editing.
(Photo: Pigs at the Roslin Institute that have been gene-edited with the goal of making them resistant to African Swine Fever virus)